| Bioactivity | Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference[1][2]. | ||||||||||||
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling diazo-labeled proteins[2][3]:1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature.2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 °C for 18 h.3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with λex=365 nm, λem=480 nm. | ||||||||||||
| Name | Fl-DIBO | ||||||||||||
| CAS | 1407523-31-2 | ||||||||||||
| Formula | C19H12O3 | ||||||||||||
| Molar Mass | 288.30 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Ke Wang, et al. A metal-free turn-on fluorescent probe for the fast and sensitive detection of inorganic azides. Bioorg Med Chem Lett. 2016 Apr 1;26(7):1651-4. [2]. Frédéric Friscourt, et al. Fluorogenic Strain-Promoted Alkyne-Diazo Cycloadditions. Chemistry. 2015 Sep 28;21(40):13996-4001. doi: 10.1002/chem.201502242. Epub 2015 Aug 18. [3]. Camille Favre, et al. Sydnone Reporters for Highly Fluorogenic Copper-Free Click Ligations. J Org Chem. 2018 Feb 16;83(4):2058-2066. |