| Bioactivity | FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols. FM-red can be used to image protein sulfhydryl groups in live cells and in vivo. FM-red also could be used to measure of the redox states of thioredoxin (Trx)[1]. |
| Invitro | FM-red (10 μM; 30 min) is suitable for imaging protein thiols in HeLa cells[1].FM-red (20 μM; 48 h) has no significant cytotoxicity in HeLa cells[1].FM-red possesses a long emission wavelength (~655 nm) and fast response (~10 min) by binding to protein thiols[1].FM-red exhibits time- and concentration-dependent fluorescence response to bovine serum albumin (BSA)[1]. |
| In Vivo | FM-red (10 μM; 30 min) could be used to image protein thiols in zebrafishes[1]. |
| Name | FM-red |
| Formula | C41H46ClN5O9 |
| Molar Mass | 788.29 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Hu G, et, al. Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe. J Mater Chem B. 2019 Apr 28;7(16):2696-2702. |