| Bioactivity | EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682). Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation[1][2]. |
| Invitro | EDTA-AM 处理有丝分裂细胞：1.收集有丝分裂细胞，并将其接种到 12 孔培养板上，该培养板具有聚赖氨酸包被的盖玻片和补充有 10% FBS 的 DMEM。2.将 EDTA-AM 溶解于 100 mM DMSO 中，将 EDTA-AM 以终浓度为 20 μM 加入到含有 0.02% Pluronic F-127 的培养基中，并将细胞进一步在 37℃ 下孵育 100 分钟。3.孵育后，计数有丝分裂细胞和间期细胞的数量。将盖玻片上的细胞固定，用 DAPI 染色，并用对苯二胺 (PPDI) 溶液 (20 mM HEPES：pH7.4， 1 mM MgCl 2，100 mM KCl，78% 甘油，1 mg/mL PPDI) 封片。 |
| Name | EDTA-AM |
| CAS | 162303-59-5 |
| Formula | C22H32N2O16 |
| Molar Mass | 580.49 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Watkins CS, et.al. Effects on K+ currents in rat cerebellar granule neurones of a membrane-permeable analogue of the calcium chelator BAPTA. Br J Pharmacol. 1996 Aug;118(7):1772-8. [2]. Maeshima K, et.al. A Transient Rise in Free Mg2+Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444-451.e6. |