| Bioactivity | DiOC7(3) (3,3'-Diheptyloxacarbocyanine iodide) is a green membrane potential probe (Ex=450-490 nm, Em=510-520 nm). DiOC7(3) can be used to quantify the vascular densities[1]. |
| In Vivo | Identification for tumour vasculature (frozen section):Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].1. The dye is administered to murine via lateral tail vein injection at a dose of 1 mg/kg/ in 50 μL volume. 2. Animals are killed 5 min after dye administration, and tumors are immediately excised and frozen at - 20°C. 3. Frozen sections 10 μm in thickness are rewarmed to room temperature, allowed to air dry. 4. Image using a microscope with 100W mercury lamp illumination and an epifluorescence condenser (excited at 430-490 nm, emitted at 510-520 nm. |
| Name | DiOC7(3) |
| CAS | 53213-83-5 |
| Formula | C31H41IN2O2 |
| Molar Mass | 600.57 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. M J Trotter, et al. Use of a carbocyanine dye as a marker of functional vasculature in murine tumours. Br J Cancer. 1989 May;59(5):706-9. |