| Bioactivity | DIBA-Cy5 is a fluorescent DIBA antagonist made up be DIBA-alkyne binding Cyanine5 fluorophores (Cy5) and polyethylene glycol (PEG) biomolecules. DIBA-Cy5 can serve as a fluorescent ligand, suitable for probe attachment through click chemistry. DIBA-Cy5 exerts a high binding affinity to type-2 mAChR (M2R) with the Kd value of 1.80 nM, can directly stain M2R receptors in the sinoatrial node of a mouse heart[1]. |
| Invitro | DIBA-Cy5 (0.01 nM-1 mM; 5 h) shows selective binding property for type-2 mAChR (M2R) over M1R, with Kd of 1.08 nM and 104.5 nM, respectively[1].DIBA-Cy5 (10 nM; 3 h) exerts the competitive binding mode with orthosteric antagonist Atropine (HY-B1205)/allosteric modulator LY2119620 (HY-15885), indicating a dualsteric binding mode of the DIBA-type antagonist to M2R[1].DIBA-Cy5 (50 nM; 16 h) results direct staining to M2R receptors in the sinoatrial node of a mouse heart. DIBA-Cy5 can be used binding assays that screen compounds for M2R as the receptor target[1]. |
| Name | DIBA-Cy5 |
| Formula | C69H89N11O12S3 |
| Molar Mass | 1360.71 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Yang H, et al. Click Chemistry-Enabled Conjugation Strategy for Producing Dibenzodiazepinone-Type Fluorescent Probes To Target M2 Acetylcholine Receptors. Bioconjug Chem. 2022 Nov 3. |