| Bioactivity | DAz-1 is a sulfonic acid probe for living cells, which can directly detect sulfonic acid-modified proteins in living cells and is cell-permeable[1]. |
| Invitro | 不同情况下 DAz-1 磺酸的检测方法[1]1. 制备 10 μM 蛋白，或 50 μg 细胞裂解液，或 200 μg 蛋白裂解液。 2. 加入 50 或 100 μM H2O2 室温反应 20 min； 然后添加 1 mM Daz-1 或 5% (v/v) DMSO，并在室温下反应 15 min。 3. 用 5 mM DTT 猝灭反应，并通过凝胶过滤除去多余的试剂。 4. 使用 4-12% SDS-PAGE 分离蛋白质，转移至 PVDF 膜，并通过 HRP-Streptavidin WB 检测。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> DAz-1 相关抗体: |
| CAS | 1112977-84-0 |
| Formula | C10H14N4O3 |
| Molar Mass | 238.24 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Leonard SE, et al. Mining the thiol proteome for sulfenic acid modifications reveals new targets for oxidation in cells. ACS Chem Biol. 2009 Sep 18;4(9):783-99. |