PeptideDB

DAPI (dihydrochloride) (solution)

CAS: 28718-90-3 F: C16H17Cl2N5 W: 350.25

DAPI dihydrochloride (4',6-Diamidino-2-phenylindole dihydrochloride) (solution) is a DAPI dye. DAPI is a fluorescent dye
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Bioactivity DAPI dihydrochloride (4',6-Diamidino-2-phenylindole dihydrochloride) (solution) is a DAPI dye. DAPI is a fluorescent dye that can bind strongly to DNA. DAPI binds to the A/T base pairs in the minor groove of double-stranded DNA (1 DAPI molecule can occupy 3 base pairs), which amplifies the fluorescence intensity by about 20 times. By observing the fluorescence intensity, DNA can be quantified. DAPI can also penetrate intact cell membranes and is often used to stain living and fixed cells[1].
CAS 28718-90-3
Formula C16H17Cl2N5
Molar Mass 350.25
Transport Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Reference [1]. Kapuscinski J. DAPI: a DNA-specific fluorescent probe[J]. Biotechnic & histochemistry, 1995, 70(5): 220-233.