| Bioactivity | D-Trehalose is a disaccharide formed by a 1,1-glycosidic bond between two α-glucose units and is widely used as a food ingredient and pharmaceutical excipient. D-Trehalose is known to protect and stabilise proteins. For long-term storage, recombinant protein solution should be diluted further with 5% D-Trehalose, Carrier Protein. |
| Invitro | D-Trehalose 是一种典型应激代谢物，能够在高温、高寒、高渗透压及干燥失水等恶劣环境条件下在细胞表面形成独特的保护膜，从而维持生命体的生命过程和生物特征。1. 稳定细胞膜和蛋白质结构2. 抗干燥3. 药用辅料，用作赋形剂4. 抑制骨胶原分解塑料管壁对蛋白有很强的吸附作用，会导致重组蛋白粘附在管壁不易分离，进而导致溶液中实际浓度偏低，最终表现为活性下降。在复溶重组蛋白时，加入载体蛋可以预先封闭塑料管壁上蛋白结合位点，避免重组蛋白粘附于管壁，从而保护蛋白活性。做无血清培养或体内实验、蛋白标记时，使用的重组蛋白中不能含有 BSA、FBS 或 HSA 等动物或人的蛋白，要想长期保存重组蛋白则可用海藻糖作为载体蛋白。为了维持一定的 pH 值跟盐类浓度，避免蛋白不稳定，不建议用水溶解载体蛋白，该溶液通常建议选择 pH 近中性、有一定缓冲能力的缓冲液，如 PBS、培养基 (RPMI1640, DMEM) 等。 |
| Name | D-Trehalose, Carrier Protein |
| CAS | 99-20-7 |
| Formula | C12H22O11 |
| Molar Mass | 342.30 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
D-Trehalose, Carrier Protein
CAS: 99-20-7 F: C12H22O11 W: 342.30
D-Trehalose is a disaccharide formed by a 1,1-glycosidic bond between two α-glucose units and is widely used as a food
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