| Bioactivity | Creatinase (Recombinant) is a hydrolase responsible for catalyzing the hydrolysis of creatine (HY-W010388) into sarcosine (HY-101037) and urea (HY-Y0271). Creatinase (Recombinant) can be used in conjunction with creatinine deiminase (HY-P2838) and sarcosine oxidase (HY-P2861) in an enzyme cascade reaction to measure creatinine (HY-B0504) levels in blood and urine. Creatinase (Recombinant) is the rate-limiting enzyme in this enzymatic cascade, and the enzymatic quantification of creatinine is an important method for evaluating kidney function[1][2]. |
| CAS | 37340-58-2 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Schumann J, et al. Stabilization of creatinase from Pseudomonas putida by random mutagenesis. Protein Sci. 1993 Oct;2(10):1612-20. [2]. Bai X, et al. Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis. Microb Cell Fact. 2020 Oct 17;19(1):194. |