Bioactivity | CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm)[1]. |
Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fixation and staining of self-assembled spheroids[1]:1. Resuspended cells are stained with CellTracker Red CMTPX and allowed to self-assemble into spheroids before aggregation. 2. Cell aggregates are fixed in 4% paraformaldehyde/PBS for 30 min with gentle inversion, then washing cells in PBS for 3 times and stained with 100 ng/mL Hoechst dye overnight prior to imaging.使用指南 (以下是我们推荐的方案,本方案仅提供指南,应根据您的具体需要进行修改)。自组装球体的固定和染色[1]:1. 用 CellTracker Red CMTPX 对重悬的细胞进行染色,并允许其在聚集之前自组装成球体。2. 将细胞聚集物在 4% 多聚甲醛/PBS 中固定 30 分钟,温和倒置,然后在 PBS 中洗涤细胞 3 次,用 100 ng/mL 的 Hoechst 染料染色过夜,然后进行成像。 |
Name | CellTracker Red CMTPX |
Formula | C42H40ClN3O4 |
Molar Mass | 686.24 |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
Reference | [1]. Van Pel DM, et al. Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture. J Cell Commun Signal. 2018 Dec;12(4):723-730. |