CellTracker Orange CMRA Dye_product_DataBase

Bioactivity	CellTracker Orange CMRA Dye is a fluorescent dye. CellTracker Orange CMRA Dye can be used for cell imaging and monitoring the movement and location of cells[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Cell viability assay[1]:1. Adds 5 μM CellTracker Orange CMRA Dye to each sample at 27-33℃ for 30 min to label sample.2. Centrifuges cells 1000 g for 10 min, resuspends cell pellet in pre-warmed 10% iFCS RPMI (3*107cells/Ml).3. Incubates cells at 27-33℃ for 30 min.4. Centrifuges cells 1000 g for 10 min and wash with pre-warmed PBS without Ca2+ and Mg2+.5. Centrifuges cells 1000 g for 10 min and cells resuspends in pre-warmed 10% iFCS RPMI.6. Measure fluorescence intensity (Ex=548 nm, Em=576 nm).
Name	CellTracker Orange CMRA Dye
Formula	C30H25Cl2NO5
Molar Mass	550.43
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Dagley MJ, et al. High-content assay for measuring intracellular growth of Leishmania in human macrophages. Assay Drug Dev Technol. 2015 Sep;13(7):389-401.

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