| Bioactivity | Cas9-IN-1 is a potent Cas9 inhibitor (IC50=7.02 μM), acting by binding to apo-Cas9 to prevent Cas9:gRNA complex formation[1]. |
| Invitro | Cas9-IN-1 (compound 85) (5 and 13 μM; overnight) can detach gRNA from preformed Cas9:gRNA complex while having weaker inhibitory activity, helping the E. coli selection strain to survive SpyCas9-induced killing[1]. |
| Name | Cas9-IN-1 |
| Formula | C17H11Cl2F3N2O3S |
| Molar Mass | 451.25 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Sang-Woo Lee et al. Identification and Optimization of Novel Small-Molecule Cas9 Inhibitors by Cell-Based High-Throughput Screening. Journal of Medicinal Chemistry 2022; 65(4), 3266-3305. |