| Bioactivity | CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive[1]. |
| Invitro | Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ROS Measurement:1. Incubate the cells according to your normal protocol.2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.3. Wash the excess probe.4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. |
| Name | CM-H2DCFDA |
| CAS | 850013-49-9 |
| Formula | C27H19Cl3O8 |
| Molar Mass | 577.79 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Oparka M, et al. Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11. [2]. Kolarova H, et al. Production of reactive oxygen species after photodynamic therapy by porphyrin sensitizers. Gen Physiol Biophys. 2008 Jun;27(2):101-5. |
CM-H2DCFDA
CAS: 850013-49-9 F: C27H19Cl3O8 W: 577.79
CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 49
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This product is for research use only, not for human use. We do not sell to patients.