| Bioactivity | CB2-H is a dual-channel fluorescent probe for the simultaneous detection of HOCl and ONOO-. CB2-H enables the concurrent detection of HOCl and ONOO- at two independent channels without spectral cross-interference and can be applied for dual-channel fluorescence imaging of endogenously produced HOCl and ONOO- in living cells and zebrafish under different stimulants[1]. |
| Invitro | ONOO- 可选择性氧化 CB2-H 的肼基，生成母染料 CB2 (Absmax/Emmax = 631/669 nm)。在 HOCl 的情况下，它对 CB2-H 的苯并吡喃部分进行亲电攻击，产生氯化产物 CB2-H-Cl，抑制探针内的 PET 过程，从而在香豆素通道上提供开启荧光响应 (Absmax/Emmax = 407/468 nm)[1]。CB2-H (5 μM; 30 min) 能够同时监测活细胞和斑马鱼中在不同刺激物下内源性产生的 HOCl 和 ONOO-[1]。 |
| Name | CB2-H |
| Formula | C29H23Br2N3O5 |
| Molar Mass | 653.32 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Huang W, et al. Rational Design of a Dual-Channel Fluorescent Probe for the Simultaneous Imaging of Hypochlorous Acid and Peroxynitrite in Living Organisms. Anal Chem. 2022 Dec 20;94(50):17485-17493. |