| Bioactivity | C12 NBD sphingomyelin is an active derivative of sphingomyelin (HY-113498) that is tagged with fluorescent C12 nitrobenzoxadiazole (C12 NBD). C12 NBD sphingomyelin can be used as a sphingomyelinase substrate for studying the metabolism and transport of sphingomyelins (Ex=470 nm, Em=525 nm)[1]. |
| Target | Sphingomyelinase |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].Assay for Sphingolipid-Degrading Enzymes (EGCase Ⅱ, SCDase and SMase): 1. Incubate amounts of enzymes with 0.1 nM dye at 37 ℃ for indicated times under following conditions.(1). 10 mM sodium acetate buffer (pH5.0) containing 0.2% Triton X-100 for EGCase.(2) 25 mM sodium phosphate buffer (pH 6.0) containing 0.1% Triton X-100 for SCDase.(3) 25 mM sodium phosphate buffer (pH 7.0) containing 0.2% Triton X-100 for SMase.2. After incubation, the solvent is evaporated and the residue is dried, dissolved in 10 μL of chloroform/methanol (2:1) and analyzed by TLC using chloroform/methanol/0.02% CaCl2 (5:4:1, v/v) as the developing solvent. 3. Degradation products and remaining substrates are separated by TLC and quantified with a chromatoscanner (excitation 470 nm, emission 525 nm) for fluorescence-labeled substrates. |
| Name | C12 NBD Sphingomyelin |
| CAS | 254117-01-6 |
| Formula | C41H73N6O9P |
| Molar Mass | 825.03 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Nakagawa, et al. Preparation of fluorescence-labeled GM1 and sphingomyelin by the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase as substrates for assay of sphingolipid-degrading enzymes and for detection of sphingolipid-binding proteins |
C12 NBD Sphingomyelin
CAS: 254117-01-6 F: C41H73N6O9P W: 825.03
C12 NBD sphingomyelin is an active derivative ofsphingomyelin (HY-113498) that is tagged with fluorescent C12 nitrobenzo
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