| Bioactivity | Boc-Val-Pro-Arg-MCA is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1]. |
| Invitro | 胰蛋白酶活性测定[2]1. 将稀释的 Trypsin 酶 (100 μL) 加入到 800 μL 的 50mM Tris-HCl 缓冲液 (pH 8.0) 中。2. 加入 100 μL 50 μM 底物 Boc-Val-Pro-Arg-MCA 可立即引发反应。3. 55°C 孵育 10 分钟。4. 加入 1.5 mL 停止剂 (甲醇:正丁醇:蒸馏水= 35:30:35,v/v/v) 停止反应。5. 用荧光光谱仪测量荧光强度 (excitation 380 nm, emission 450 nm)。 |
| Name | Boc-Val-Pro-Arg-AMC |
| CAS | 65147-04-8 |
| Sequence | {Boc}-Val-Pro-Arg-{AMC} |
| Shortening | {Boc}-VPR-{AMC} |
| Formula | C31H45N7O7 |
| Molar Mass | 627.73 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204. [2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358. |