Biotin-C2-maleimide_product_DataBase

Bioactivity	Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule[1][2].
Invitro	Biotin-C2-maleimide can act bioconjugation with 5 eq. SUMO-1 probes[1].1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 µM) at room temperature.2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4).
Name	Biotin-C2-maleimide
CAS	139554-72-6
Formula	C16H22N4O4S
Molar Mass	366.44
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Benjamin GD, et al. Antibody conjugates and methods of making the antibody conjugates: United States, US20160361436A1. 2016-12-15. [2]. Brüninghoff K, Wulff S, Dörner W, Geiss-Friedlander R, Mootz HD. A Photo-Crosslinking Approach to Identify Class II SUMO-1 Binders. Front Chem. 2022 May 30;10:900989.

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