| Bioactivity | Biotin-16-dUTP (Biotin-16-deoxyuridine-5'-triphosphate) can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP can be used to produce biotinylated DNA probes in a variety of assay applications[1][2]. |
| Invitro | Biotin-16-dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis[1].Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin[2]. |
| Name | Biotin-16-dUTP |
| CAS | 86303-26-6 |
| Formula | C32H52N7O18P3S |
| Molar Mass | 947.78 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4. [2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11. |
Biotin-16-dUTP
CAS: 86303-26-6 F: C32H52N7O18P3S W: 947.78
Biotin-16-dUTP (Biotin-16-deoxyuridine-5'-triphosphate) can be used to replace its natural counterpart dTTP by enzymatic
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