| Bioactivity | BTS (N-Benzyl-p-toluenesulfonamide) is a potent and selective inhibitor of skeletal muscle myosin II subfragment 1 (S1) ATPase activity, with an IC50s of ~5 µM for actin- and Ca2+-stimulated myosin S1 ATPase. BTS specifically inhibits the contraction of fast skeletal muscle fibers[1][2]. | ||||||||||||
| Target | IC50: ~5 µM (skeletal muscle myosin II S1 ATPase) | ||||||||||||
| Invitro | BTS (2-12 μM) inhibits the Ca2+-stimulated ATPase activity of myosin S1 in the absence of actin, with an IC50 of ~5 µM[1].BTS (2-20 μM) reversibly inhibits the gliding motility rate of heavy meromyosin (HMM)[1].BTS (100 μM) releases myosin from actin in the presence of ADP[1].BTS (0-20 μM) reversibly inhibits isometric Ca2+-activated tension in skinned skeletal muscle fibres from rabbit and frog, with IC50s of ~3 µM and 1 µM, respectively[1]. | ||||||||||||
| Name | BTS | ||||||||||||
| CAS | 1576-37-0 | ||||||||||||
| Formula | C14H15NO2S | ||||||||||||
| Molar Mass | 261.34 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Cheung A, et al. A small-molecule inhibitor of skeletal muscle myosin II. Nat Cell Biol. 2002 Jan;4(1):83-8. [2]. Shaw MA, et, al. Mechanism of inhibition of skeletal muscle actomyosin by N-benzyl-p-toluenesulfonamide. Biochemistry. 2003 May 27;42(20):6128-35. |