| Bioactivity | BTCy is a near-infrared (NIR) fluorescence probe with polarity-responsive and cell plasma membrane-targeting properties. BTCy can be used for in vivo imaging of tumor tissue (λex = 561 nm, λem = 600-700 nm)[1]. |
| Invitro | BTCy (0-50 μM; 24 h) 具有低细胞毒性，对活细胞具有生物相容性[1]。BTCy 具有良好的荧光稳定性，能够选择性地检测极性变化[1]。BTCy (10 μM) 染色 HeLa 和 HepG2 细胞。染色 0.5-5 h 后，细胞质膜特异性强亮，BTCy在细胞质膜上的荧光强度在 2 h 后略有降低[1]。BTCy (0.1-10 μM; 30 min) 在 HeLa 细胞中，即使在 100 nM 的低浓度染色也显示细胞质膜上有特异性荧光[1]。BTCy (0.1-10 μM;30 min) 可用于监测铁死亡过程中细胞膜极性变化[1]。 |
| In Vivo | BTCy (0.5 mg/mL; 25 μL; in situ injection) 携带 4T1 肿瘤的小鼠中，与正常组织相比，在肿瘤区域表现出显著强且长时间持续的荧光信号[1]。 |
| Name | BTCy |
| Formula | C28H25BrN2OS2 |
| Molar Mass | 549.54 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Liu J, et al. Monitoring Cell Plasma Membrane Polarity by a NIR Fluorescence Probe with Unexpected Cell Plasma Membrane-Targeting Ability. ACS Omega. 2022 Dec 9;7(50):46891-46899. |