| Bioactivity | BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1]. Storage: protect from light. | |||||||||
| Invitro | BODIPY-cholesterol assayTo prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM[1].Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration[1].Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C[1].The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment[1]. | |||||||||
| Name | BODIPY-Cholesterol | |||||||||
| CAS | 878557-19-8 | |||||||||
| Formula | C36H51BF2N2O | |||||||||
| Molar Mass | 576.61 | |||||||||
| Appearance | Solid | |||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | |||||||||
| Storage |
|
|||||||||
| Reference | [1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28. [2]. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49. |
BODIPY-Cholesterol
CAS: 878557-19-8 F: C36H51BF2N2O W: 576.61
BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY gr
Sales Email：peptidedb@qq.com
This product is for research use only, not for human use. We do not sell to patients.