Astrophloxine_product_DataBase

Bioactivity	Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice[1].
Target	Antiparallel dimer
Invitro	Astrophloxine binds Aβ dimers with stronger fluorescence intensity (A.U.) than binding Aβ monomers[1].Protocol for CSF analyses[1]:1.Prepare 1 μL of CSF samples with 1 μL of 10X protease inhibitors and diluted the mixture with 8 μL of binding buffer. Binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride, 10 mM EDTA; pH 7.2).2.Dilute Astrophloxine to 0.5 μM with binding buffer.3.Add individual samples and dye to the wells of a 96-well half-area black microplate with a sample to dye ratio of 1:3.Detect at λex=540 nm/λem=570 nm.
In Vivo	Astrophloxine shows more fluorescence (A.U.) in APP/PS1 mice CSF rather in WT mice, and is able to indicate soluble Aβ oligomers in CSF and insoluble plaques in brain tissue[1].
Name	Astrophloxine
CAS	14696-39-0
Formula	C27H33IN2
Molar Mass	512.47
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Lee JC, et al. Discovery of Chemicals to Either Clear or Indicate Amyloid Aggregates by Targeting Memory-Impairing Anti-Parallel Aβ Dimers. Angew Chem Int Ed Engl. 2020 Jul 6;59(28):11491-11500.

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