| Bioactivity | Anti-inflammatory agent 64 (compound 4b) inhibits the secretion of IL-6 and TNF-α. Anti-inflammatory agent 64 has antioxidant and anti-inflammatory activity in vitro and in vivo. Anti-inflammatory agent 64 can effectively reduce paw edema [1]. |
| Invitro | Anti-inflammatory agent 64 (2 μM, 4 h) 在经过 H2O2 ( 0.4 mM) 刺激的 RAW264.7 细胞中能够显著保护细胞，促进细胞增殖[1]。Anti-inflammatory agent 64 (2 μM, 24 h) 在经过 LPS (1 μg/mL) 刺激的 RAW264.7 细胞中能够抑制一氧化氮 (NO) (IC50=0.9 μM) 的产生，具有抗炎作用[1]。 Anti-inflammatory agent 64 (0.125-2 μM, 4-24 h) 在经过 Rot (5 μM) 和 LPS (1 μM/mL) 刺激的 RAW264.7 细胞中能够显著降低 ROS 的蛋白表达，提高 HO-1 的蛋白表达，具有抗氧化作用[1]。 Anti-inflammatory agent 64 (0.5-2 μM, 24 h) 能够以剂量依赖的方式抑制 IL-6 (IC50=1.73 μM) 和 TNF-α ( IC50=1.52 μM)[1]。Anti-inflammatory agent 64 (0.5-2 μM) 能够抑制 iNOS 的蛋白表达[1]。Anti-inflammatory agent 64 (0.5-2 μM) 在经过 LPS (1 μg/mL) 刺激的 RAW264.7 细胞中通过抑制 IκBα 的降解和磷酸化来抑制来抑制了 NF-κB 通路的活性[1]。 0 --> Anti-inflammatory agent 64 相关抗体: Cell Viability Assay[1] Cell Line: |
| In Vivo | Anti-inflammatory agent 64 (1-30 mg/kg, 腹腔注射, 灌胃给药, 单剂量) 在角叉菜胶 (Cg) (HY-125474) 刺激的小鼠足跖水肿模型具有抗炎活性和抗氧化活性[1]。 Animal Model: |
| Name | Anti-inflammatory agent 64 |
| Formula | C20H21ClN2O3 |
| Molar Mass | 372.85 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Liu Y, et al. Synthesis of cinnamoyl tethered indoline derivatives with anti-inflammatory and antioxidant activities[J]. European Journal of Medicinal Chemistry, 2023: 115936. |