Ac-WEHD-AFC_product_DataBase

Bioactivity	Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Caspase-1 Fluorogenic Activity Assay[1]:1. Incubate the cells according to your normal protocol.2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.
Name	Ac-WEHD-AFC
CAS	210344-99-3
Shortening	Ac-WEHD-{AFC}
Formula	C38H37F3N8O11
Molar Mass	838.74
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Katherine Labbé, et al. Cellular inhibitors of apoptosis proteins cIAP1 and cIAP2 are required for efficient caspase-1 activation by the inflammasome. Immunity

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