PeptideDB

Ac-VEID-AMC

CAS: 219137-97-0 F: C32H43N5O11 W: 673.71

Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=3
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Bioactivity Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].
Invitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Enzyme activity (rabbit osteoclasts for example)[1]:1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell).2. Put treated cells into 4.5 mL lysis buffer.3. Each lysate is divided into thirds, then incubate with 40 µM Ac-VEID-AMC at 37°C for 1 h.4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)
Name Ac-VEID-AMC
CAS 219137-97-0
Formula C32H43N5O11
Molar Mass 673.71
Transport Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.