Bioactivity | Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1]. |
Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Enzyme activity (rabbit osteoclasts for example)[1]:1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell).2. Put treated cells into 4.5 mL lysis buffer.3. Each lysate is divided into thirds, then incubate with 40 µM Ac-VEID-AMC at 37°C for 1 h.4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm) |
Name | Ac-VEID-AMC |
CAS | 219137-97-0 |
Formula | C32H43N5O11 |
Molar Mass | 673.71 |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |