| Bioactivity | Ac-DEVD-CMK (Caspase-3 Inhibitor III) TFA is a selective and irreversible caspase-3 inhibitor. Ac-DEVD-CMK TFAsignificantly inhibits apoptosis induced by high levels of glucose or 3,20-dibenzoate (IDB; HY-137295). Ac-DEVD-CMK TFA can be used in a variety of experimental approaches to inhibit apoptosis[1][2][3]. | ||||||
| Invitro | Ac-DEVD-CMK TFA (100 μM; 24 h) 抑制 IDB 诱导的细胞凋亡[3]。Ac-DEVD-CMK TFA (10 μM; 36 h) 抑制人咽鳞癌 FaDu 和 Detroit 562 细胞系中柠檬酸盐 (10 mM) 诱导的 p21 裂解和 G2/M 积累[4]。 Western Blot Analysis[4] Cell Line: | ||||||
| In Vivo | Ac-DEVD-CMK TFA (Caspase-3 Inhibitor III；25 mg/kg；腹腔注射；单剂量；APAP 后 3 小时) 显着减弱对乙酰氨基酚 (APAP；HY-66005) 诱导的肝损伤 (AILI)[5]。Ac-DEVD-CMK TFA (25 mg/kg；ip；单剂量) 显著减轻易感 Sdc1−/− 小鼠中 APAP 诱导的肝损伤 (AILI)[6]。 Animal Model: | ||||||
| Name | Ac-DEVD-CMK TFA | ||||||
| Formula | C23H32ClF3N4O13 | ||||||
| Molar Mass | 664.97 | ||||||
| Appearance | Solid | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture and light, under nitrogen
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) |
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| Reference | [1]. Lu Cai, et al. Hyperglycemia-induced apoptosis in mouse myocardium: mitochondrial cytochrome C-mediated caspase-3 activation pathway. Diabetes. 2002 Jun;51(6):1938-48. [2]. M M Mocanu, et al. Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury. Br J Pharmacol. 2000 May;130(2):197-200. [3]. M Blanco-Molina, et al. Ingenol esters induce apoptosis in Jurkat cells through an AP-1 and NF-kappaB independent pathway. Chem Biol. 2001 Aug;8(8):767-78. [4]. Hung KC, et al. Citrate-Induced p85α⁻PTEN Complex Formation Causes G2/M Phase Arrest in Human Pharyngeal Squamous Carcinoma Cell Lines. Int J Mol Sci. 2019 Apr 29;20(9):2105. [5]. Eon Jeong Nam, et al. Syndecan-1 limits the progression of liver injury and promotes liver repair in acetaminophen-induced liver injury in mice. Hepatology. 2017 Nov;66(5):1601-1615. [6]. Nam EJ, et al. Syndecan-1 limits the progression of liver injury and promotes liver repair in acetaminophen-induced liver injury in mice. Hepatology. 2017 Nov;66(5):1601-1615. |