| Bioactivity | Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm). | ||||||
| Invitro | After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1]. | ||||||
| Name | Ac-DEVD-AFC | ||||||
| CAS | 201608-14-2 | ||||||
| Sequence | N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoroMethylcoumarin | ||||||
| Shortening | Ac-DEVD-7-amido-4-trifluoroMethylcoumarin | ||||||
| Formula | C30H34F3N5O13 | ||||||
| Molar Mass | 729.61 | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |