| Bioactivity | AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol[1][2][3]. |
| Target | IC50: 13 nM (DHCR7)1 |
| Invitro | AY 9944 (1 μg/mL; 15 h) affects types of free sterol in keratinocytes[2]. Cell Viability Assay[2] Cell Line: |
| In Vivo | AY 9944 (7.5 mg/kg; i.h. every 6 days once from postnatal day 2 to postnatal day 20) reduces brain cholesterol until 400 days after stop treatment[3]. Animal Model: |
| Name | AY 9944 |
| CAS | 366-93-8 |
| Formula | C22H30Cl4N2 |
| Molar Mass | 464.30 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Moebius FF, et al. Molecular cloning and expression of the human delta7-sterol reductase. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1899-902. [2]. Nemanic MK, et al. In vitro synthesis of vitamin D-3 by cultured human keratinocytes and fibroblasts: action spectrum and effect of AY-9944. Biochim Biophys Acta. 1985 Sep 6;841(3):267-77. [3]. Cortez MA, et al. Brain sterols in the AY-9944 rat model of atypical absence seizures. Epilepsia. 2002 Jan;43(1):3-8. |