| Bioactivity | AKTide-2T TFA is an excellent in vitro substrate for AKT and shows competitive inhibition of histone H2B phosphorylation with a Ki of 12 nM. AKTide-2T TFA mimics the optimal phosphorylation sequence of Akt and is an inhibitory peptide with the wildtype AKTide lacking Thr in the S22 position[1]. |
| Invitro | AKTide-2T TFA mimics the optimal phosphorylation sequence of Akt, and inhibits Akt phosphorylated Hsp27 by addition of 20 μM Akt inhibitory peptide[1]. |
| Name | AKTide-2T TFA |
| Sequence | Ala-Arg-Lys-Arg-Glu-Arg-Thr-Tyr-Ser-Phe-Gly-His-His-Ala |
| Shortening | ARKRERTYSFGHHA |
| Formula | C76H115F3N28O22 |
| Molar Mass | 1829.89 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. T Obata,et al. Peptide and protein library screening defines optimal substrate motifs for AKT/PKB. J Biol Chem. 2000 Nov 17;275(46):36108-15. [2]. Madhavi J Rane, et al. Heat shock protein 27 controls apoptosis by regulating Akt activation. J Biol Chem. 2003 Jul 25;278(30):27828-35. |