| Bioactivity | AAA-10 formic is an orally active gut bacterial bile salt hydrolases (BSH) inhibitor, with IC50s of 10 nM, 80 nM against B. theta rBSH and B. longum rBSH, respectively[1]. |
| Target | IC50: 10 nM (B. theta rBSH), 80 nM (B. longum rBSH) |
| Invitro | AAA-10 (100 μM; 24 h) formic inhibits BSH activity in bacterial cultures, with IC50s of 74 nM, 901 nM for Gram-negative and Gram-positive bacteria, respectively[1]. AAA-10 (20 μM; 2 h) formic significantly inhibits deconjugation of glycochenodeoxycholic acid-d4 or taurocholic acid-d4 substrates of human feces[1]. |
| In Vivo | AAA-10 (30 mg/kg; orally gavage daily for 5 days) formic decreases the abundances of deoxycholic acid (DCA) and lithocholic acid (LCA) in mice feces starting in day 2-5[1].AAA-10 (30 mg/kg) formic displays high colonic exposure and low gut permeability[1]. Animal Model: |
| Name | AAA-10 formic |
| Formula | C26H43FO7S |
| Molar Mass | 518.68 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Adhikari AA, et al. A Gut-Restricted Lithocholic Acid Analog as an Inhibitor of Gut Bacterial Bile Salt Hydrolases. ACS Chem Biol. 2021;16(8):1401-1412. |