740 Y-P TFA_product_DataBase

Bioactivity	740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone[1].
Invitro	740 Y-P TFA (50 μg/ml; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 or wortmannin potently inhibits the mitogenic response stimulated by the 740 Y-P TFA peptide[1].740 Y-P TFA (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[1].740 Y-P TFA (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells[2]. Western Blot Analysis[2] Cell Line:
Name	740 Y-P TFA
Shortening	RQIKIWFQNRRMKWKKSDGG-{PO2Y}-MDMS
Formula	C143H223N43F3O41PS3
Molar Mass	3384.72
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.

PeptideDB