| Bioactivity | 5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. |
| Name | 5(6)-FITC |
| CAS | 27072-45-3 |
| Formula | C21H11NO5S |
| Molar Mass | 389.38 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| Reference | [1]. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26. [2]. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63. [3]. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9. |