Bioactivity | 4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate) is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-Nitrophenyl palmitate, 4-nitrophenol is released, which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipase. |
CAS | 1492-30-4 |
Formula | C22H35NO4 |
Molar Mass | 377.52 |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
Reference | [1]. Gupta, et al. Simplified para-nitrophenyl palmitate assay for lipases and esterases. Anal. Biochem. 311(1), 98-99 (2002). |