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4-Methylumbelliferyl-β-D-glucuronide hydrate

CAS: 881005-91-0 F: C16H18O10 W: 370.31

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl
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Bioactivity 4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli[1][2][3].
Invitro 4-Methylumbelliferyl-β-D-glucuronide hydrate (156 µM-5 mM;30 min) 在 37℃ 下释放 4-甲基伞形酮,用于酶促反应[2]。4-Methylumbelliferyl-β-D-glucuronide hydrate (50 µg/mL;24 h) 在 TSA 培养基中用于检测大肠杆菌数[3]。
Name 4-Methylumbelliferyl-β-D-glucuronide hydrate
CAS 881005-91-0
Formula C16H18O10
Molar Mass 370.31
Appearance Solid
Transport Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Reference [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5. [2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4. [3]. Villari P, et al. An evaluation of the use of 4-methylumbelliferyl-beta-D-glucuronide (MUG) in different solid media for the detection and enumeration of Escherichia coli in foods. Lett Appl Microbiol. 1997 Apr;24(4):286-90.