| Bioactivity | DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance [1][2]. |
| Invitro | HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2]. |
| Name | 3x DYKDDDDK Tag TFA |
| Shortening | DYKDDDDKDYKDDDDKDYKDDDDK |
| Formula | C125H177F3N30O60 |
| Molar Mass | 3116.90 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
3x DYKDDDDK Tag TFA
CAS: F: C125H177F3N30O60 W: 3116.90
DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (A
Data collection：peptidedb@qq.com
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