| Bioactivity | 3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[1]. | ||||||||||||
| Invitro | 3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].3-Methyladenine induces cell death is independent of autophagy inhibition[2].3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].Note:在体外细胞培养实验中，3-MA 常用的浓度为 0.5-10 mM，所以优先推荐直接称取本次实验所需粉末量，用培养基完全溶解，0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用，尽快用完。 Cell Viability Assay[2] Cell Line: | ||||||||||||
| Name | 3-Methyladenine | ||||||||||||
| CAS | 5142-23-4 | ||||||||||||
| Formula | C6H7N5 | ||||||||||||
| Molar Mass | 149.15 | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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