| Bioactivity | 3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs. |
| CAS | 3318-43-2 |
| Formula | C8H6NNa2O4P |
| Molar Mass | 257.09 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Tsou, et al. A new colorimetric method for the determination of alkaline phosphatase with indoxyl phosphate. Anal. Biochem. 11(1), 54-64 (1965). |
3-Indoxyl phosphate sodium
CAS: 3318-43-2 F: C8H6NNa2O4P W: 257.09
3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleav
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