| Bioactivity | (Rac)-Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins[1][2]. |
| Invitro | (Rac)-Tris-NTA, which in presence of Ni2+ ions can interact with a His10-tag fused to the intracellular C terminus of the receptor. Whereas the (Rac)-Tris-NTA group shows an affinity Kd to bind the His10-tag of ~10-30 nM[1]. |
| Name | (Rac)-Tris-NTA |
| CAS | 1146592-99-5 |
| Formula | C43H68N8O22 |
| Molar Mass | 1049.04 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Moritz Pfreundschuh, et al. Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nat Commun. 2015 Nov 12;6:8857. [2]. Luyao Liu, et al. Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips. Anal Chim Acta. 2021 Jul 25;1170:338625. |