| CAS | 158584-09-9 |
| Sequence | Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 |
| Sequence Single | Mca-RPKPVE-Nva-WRK(Dnp)-NH2 |
| Molecular Formula | C78H110N22O20 |
| Molecular Weight | 1675.87 |
| Technology | Synthetic |
| Storage | -20°C, avoid light, cool and dry place |
| Application | Cancer Research|Inflammation Research |
| Description | NFF-3 is a FRET substrate with the sequence of Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2, it was hydrolyzed rapidly by stromelysin 1 (MMP-3) with kcat/Km= 218000 M-1s-1 and very slowly by gelatinase B (MMP-9) (kcat/Km= 10100 M-1s-1). There was no hydrolysis by MMP-1 and MMP-2. |
| References | 1. Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3). H.Nagase et al., J. Biol. Chem., 269, 20952 (1994) |