PeptideDB

NFF-2

CAS No.: 158584-08-8

NFF-2 is a FRET substrate with the sequence of Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2, it was hydrolyzed 6
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CAS 158584-08-8
Sequence Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2
Sequence Single Mca-RPKPYA-Nva-WMK(Dnp)-NH2
Molecular Formula C79H105N19O19S
Molecular Weight 1656.89
Technology Synthetic
Storage -20°C, avoid light, cool and dry place
Application Cancer Research|Inflammation Research
Description NFF-2 is a FRET substrate with the sequence of Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2, it was hydrolyzed 60 times more rapidly by stromelysin 1 (MMP-3) (kcat/Km= 59400 M-1s-1) than by interstitial collagenase (MMP-1). However, it showed little discrimination between MMP-3, gelatinase A (MMP-2) (kcat/Km= 54000 M-1s-1), and gelatinase B (MMP-9) (kcat/Km= 55300 M⁻¹s⁻¹). Metalloelastase (MMP-12) digested NFF-2 with a kcat/Km of 243000 M-1s-1.