Mca-(Ala7,Lys(Dnp)9)-Bradykinin Matrix Metalloproteinase Catalog

CAS	323577-36-2
Sequence	Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Sequence Single	Mca-RPPGFSAFK(Dnp)-OH
Molecular Formula	C66H81N15O19
Molecular Weight	1388.46
Synonyms	Substrate V
Technology	Synthetic
Storage	-20°C, avoid light, cool and dry place
Application	Cancer Research\|Cardiovascular System & Diseases\|Inflammation Research
Description	Mca-(Ala7,Lys(Dnp)9)-Bradykinin also called substrate V, Mca-RPPGFSAFK(Dnp), is a very sensitive internally quenched fluorescent substrate for endothelin-converting enzyme-1 (ECE-1) a membrane-bound zinc metallopeptidase that is related to neprilysin in amino acid sequence. The kcat/Km value for the hydrolysis by ECE-1 was 1.9 x 10⁷ M-1s-1 and 1.7 x 10⁶ M-1s-1 for the hydrolysis by neprilysin. This FRET substrate was hydrolyzed rapidly by stromelysin 1 (MMP-3) with kcat/Km= 218000 M⁻¹s⁻¹ and very slowly by gelatinase B (MMP-9) (kcat/Km= 10100 M-1s-1). There was no hydrolysis by MMP-1 and MMP-2. Mca-(Ala7,Lys(Dnp)9)-Bradykinin has also found use as substrate for insulin-degrading enzyme (IDE) and ACE2.
References	1. 2, 5-diketopiperazines (cyclic dipeptides) in beef: identification, synthesis, and sensory evaluation. M.Z.Chen et al., J. Food Sci., 74, C100 (2009) 2. Immunocapture-based fluorometric assay for the measurement of insulin-degrading enzyme activity in brain tissue homogenates. J.M.Miners et al., J. Neurosci. Methods, 169, 177 (2008)

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