α2β1 Integrin Ligand Peptide TFA interacts with α2β1 integrin receptors on the cell membrane surface and enters cells to mediate extracellular signals. α2β1 Integrin Ligand Peptide is a potential collagen receptor blocker (antagonist).

Physicochemical Properties

Molecular Formula	C??H??F?N?O??
Molecular Weight	504.37
Related CAS #	α2β1 Integrin Ligand Peptide;134580-64-6
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

α2β1 Integrin Ligand Peptide (DGEA) TFA (0.015-2.0 mM) has no effect on CRW-8 infectivity but suppresses SA11, RRV, and Wa infection in a dose-dependent manner [1]. At doses ≥0.5 mM, α2β1 Integrin Ligand Peptide TFA causes calcium mobilization in osteoblasts and fibroblasts in a tyrosine kinase-dependent manner [1]. At 1.0 mM, α2β1 Integrin Ligand Peptide TFA can no longer prevent SA11 binding [1].

References

[1]. Graham KL, et al. Effects on rotavirus cell binding and infection of monomeric and polymeric peptides containing alpha2beta1 and alphaxbeta2 integrin ligand sequences. J Virol. 2004 Nov;78(21):11786-97.

Solubility Data

Solubility (In Vitro)	May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
Solubility (In Vivo)	Solubility in Formulation 1: 20 mg/mL (39.65 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9827 mL	9.9134 mL	19.8267 mL
	5 mM	0.3965 mL	1.9827 mL	3.9653 mL
	10 mM	0.1983 mL	0.9913 mL	1.9827 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.