VUT-MK142 is a novel, potent and cardiomyogenic agent promoting the differentiation of pre-cardiac mesoderm into cardiomyocytes, which may be useful to differentiate stem cells into cardiomyocytes for cardiac repair.

Physicochemical Properties

Molecular Formula	C17H22N4O
Molecular Weight	298.382783412933
Exact Mass	298.179
CAS #	1313491-22-3
PubChem CID	53261094
Appearance	White to off-white solid powder
Density	1.2±0.1 g/cm3
Boiling Point	499.9±40.0 °C at 760 mmHg
Flash Point	256.1±27.3 °C
Vapour Pressure	0.0±1.3 mmHg at 25°C
Index of Refraction	1.638
LogP	5.35
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	22
Complexity	314
Defined Atom Stereocenter Count	0
SMILES	O(C)C1C=CC(=CC=1)NC1=CC(=NC=N1)NC1CCCCC1
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The cardiomyogenic effects of VUT-MK142 on various cell types are promising. It was observed that VUT-MK142 significantly affected P19 and C2C12 cells. Treatment with VUT-MK142 led to a stronger upregulation of ANF expression than CgC [1]. After 7 days of treatment, VUT-MK142 (1 μM) significantly (p < 0.05) increased the luciferase signal in P19 cells by 3.1 ± 0.3 luciferase (n = 5) times [1].
References	[1]. Moumita Koley, et al. VUT-MK142 : a new cardiomyogenic small molecule promoting the differentiation of pre-cardiac mesoderm into cardiomyocytes. Medchemcomm. 2013 Aug 1;4(8):1189-1195.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~83.79 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (8.38 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.38 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3514 mL	16.7572 mL	33.5143 mL
	5 mM	0.6703 mL	3.3514 mL	6.7029 mL
	10 mM	0.3351 mL	1.6757 mL	3.3514 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.