VEGFR-IN-1 (compound 3) is a potent angiogenesis inhibitor (antagonist) with IC50 of 0.02, 0.18, 0.24, 7.3, and 7 for KDR, Flt-1, c-Kit, EGF-R, and c-Src, respectively. µM.

Physicochemical Properties

Molecular Formula	C19H16CLN3O
Molecular Weight	337.80
Exact Mass	337.098
CAS #	269390-69-4
PubChem CID	9797919
Appearance	White to off-white solid powder
LogP	4.745
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	5
Heavy Atom Count	24
Complexity	392
Defined Atom Stereocenter Count	0
InChi Key	GGPZCOONYBPZEW-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H16ClN3O/c20-15-5-7-16(8-6-15)23-19(24)17-3-1-2-4-18(17)22-13-14-9-11-21-12-10-14/h1-12,22H,13H2,(H,23,24)
Chemical Name	N-(4-chlorophenyl)-2-(pyridin-4-ylmethylamino)benzamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	KDR 0.02 μM (IC50) Flt-1 0.18 μM (IC50) EGFR 0.24 μM (IC50)
References	[1]. Identification of a new chemical class of potent angiogenesis inhibitors based on conformational considerations and database searching. Bioorg Med Chem Lett. 2003;13(18):2967-2971.

Solubility Data

Solubility (In Vitro)

DMSO: 125 mg/mL (370.04 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.08 mg/mL (6.16 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9603 mL	14.8017 mL	29.6033 mL
	5 mM	0.5921 mL	2.9603 mL	5.9207 mL
	10 mM	0.2960 mL	1.4802 mL	2.9603 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.