UBX1325 is a Bcl-xL inhibitor that promotes apoptosis of senescent cells. UBX1325 is a potent anti-aging agent for the study of age-related eye diseases like diabetic macular edema (DME), age-related macular degeneration (AMD) and diabetic retinopathy (DR).

Physicochemical Properties

Molecular Formula	C53H59CLF3N6O10PS3
Molecular Weight	1159.68674015999
Exact Mass	1158.283
CAS #	2271269-01-1
PubChem CID	147562879
Appearance	White to light yellow solid powder
LogP	7.6
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	19
Rotatable Bond Count	20
Heavy Atom Count	77
Complexity	2160
Defined Atom Stereocenter Count	1
SMILES	N1(C(C)C)C(C2=CC=C(Cl)C=C2)=C(C2=CC=CC(N3CCN(C4=CC=C(NS(C5=CC=C(N[C@@H](CSC6=CC=CC=C6)CCN6CCC(OP(O)(O)=O)CC6)C(S(C(F)(F)F)(=O)=O)=C5)(=O)=O)C=C4)CC3)=C2)C(C(O)=O)=C1C
InChi Key	FSZILRQCEAUYPL-VQJSHJPSSA-N
InChi Code	InChI=1S/C53H59ClF3N6O10PS3/c1-35(2)63-36(3)49(52(64)65)50(51(63)37-12-14-39(54)15-13-37)38-8-7-9-43(32-38)62-30-28-61(29-31-62)42-18-16-40(17-19-42)59-77(71,72)46-20-21-47(48(33-46)76(69,70)53(55,56)57)58-41(34-75-45-10-5-4-6-11-45)22-25-60-26-23-44(24-27-60)73-74(66,67)68/h4-21,32-33,35,41,44,58-59H,22-31,34H2,1-3H3,(H,64,65)(H2,66,67,68)/t41-/m1/s1
Chemical Name	5-(4-chlorophenyl)-2-methyl-4-[3-[4-[4-[[4-[[(2R)-1-phenylsulfanyl-4-(4-phosphonooxypiperidin-1-yl)butan-2-yl]amino]-3-(trifluoromethylsulfonyl)phenyl]sulfonylamino]phenyl]piperazin-1-yl]phenyl]-1-propan-2-ylpyrrole-3-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Intravitreal (IVT) injection of UBX1325 reduced anti-apoptotic Bcl-xL:Bim complexes in the retina of C57BL/6 mice model of oxygen-induced retinopathy (OIR) [1]. In mice with streptozotocin (STZ)-induced retinopathy, UBX1325 can lower retinal vascular permeability and enhance retinal neovascularization [1].
References	[1]. UBX1325, a small molecule inhibitor of Bcl-xL, attenuates vascular dysfunction in two animal models of retinopathy. Investigative Ophthalmology & Visual Science June 2021,Volume 62, Issue 8.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~86.23 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.8623 mL	4.3115 mL	8.6230 mL
	5 mM	0.1725 mL	0.8623 mL	1.7246 mL
	10 mM	0.0862 mL	0.4311 mL	0.8623 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.