TrkA-IN-3 is a potent and subselective allosteric inhibitor of TrkA with IC50 of 22.4 nM. TrkA-IN-3 is 8000-fold selective for TrkA over TrkB and TrkC. TrkA-IN-3 may be utilized to study pain.

Physicochemical Properties

Molecular Formula	C24H17F3N4O3
Molecular Weight	466.41
CAS #	3026111-73-6
PubChem CID	166176996
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	5
Heavy Atom Count	34
Complexity	731
Defined Atom Stereocenter Count	0
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	TrkA 22.4 nM (IC50)
ln Vitro	Compound 5 (TrkA-IN-3) showed 73.9% and 64.8% inhibitory rates against TrkA at 1 μM and 0.1 μM, respectively [1].
References	[1]. Design, development and evaluation of a prodrug-type TrkA-selective inhibitor with antinociceptive effects in vivo. Eur J Med Chem. 2023 Jan 5;245(Pt 2):114901.

Solubility Data

Solubility (In Vitro)

DMSO :~50 mg/mL (~107.20 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1440 mL	10.7202 mL	21.4404 mL
	5 mM	0.4288 mL	2.1440 mL	4.2881 mL
	10 mM	0.2144 mL	1.0720 mL	2.1440 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.