TRFS-red is a red fluorescence emission shut-off probe selective for thioredoxin reductase (TrxR). TRFS-red has higher response rate and sensitivity. TRFS-red can be applied to live cell imaging.

Physicochemical Properties

Molecular Formula	C28H28CLN3O11S2
Molecular Weight	682.12
Appearance	Brown to black solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In living HeLa cells, TRFS-red (1 μM; 30-120 min) produces a red fluorescence signal, with the majority of the red fluorescence occurring in the cytosol[1]. In TEBU, TRFS-red (10 μM) has a maximum absorption at about 530 nm.(pH 7.4) with 50 mM Tris-HCl and 1 mM EDTA[1]. A dependable fluorescence signal is displayed by TRFS-red in the pH range of 5.5–8.5[1].
References	[1]. A fast response and red emission probe for mammalian thioredoxin reductase. Chem Commun (Camb). 2016 Oct 4;52(81):12060-12063.

Solubility Data

Solubility (In Vitro)

DMSO :~5 mg/mL (~7.33 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.5 mg/mL (0.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.4660 mL	7.3301 mL	14.6602 mL
	5 mM	0.2932 mL	1.4660 mL	2.9320 mL
	10 mM	0.1466 mL	0.7330 mL	1.4660 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.