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Physicochemical Properties
| Exact Mass | 380.092 |
| CAS # | 62669-70-9 |
| PubChem CID | 9929799 |
| Appearance | Pink to red solid powder |
| Melting Point | 235 °C |
| LogP | 5.535 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 3 |
| Heavy Atom Count | 27 |
| Complexity | 706 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | O1C2=C([H])/C(/C([H])=C([H])C2=C(C2=C([H])C([H])=C([H])C([H])=C2C(=O)OC([H])([H])[H])C2C([H])=C([H])C(=C([H])C1=2)N([H])[H])=N/[H] |
| InChi Key | TUFFYSFVSYUHPA-UHFFFAOYSA-M |
| InChi Code | InChI=1S/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11H,22-23H2,1H31H/q+1/p-1 |
| Chemical Name | Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride |
| Synonyms | Rhodamine 123 R 22420 R 302 RH 123. |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | The rhodamine 123 working solution is prepared. 1.1 Making the stock solution Take 1 milligram of rhodamine 123 and dissolve it in 525 μL DMSO to make a 5 mM stock solution. 1.2 Making the working solution for rhodamine 123 To create a working solution ranging from 1 to 20 μM, utilize serum-free cell culture medium or PBS stock solution. Note: The concentration of the Rhodamine 123 working solution should be adjusted based on the specific circumstances. 2. The staining of cells In a 6-well plate, 2.1 suspended cells a. Discard the supernatant after centrifuging at 1000 g for three to five minutes at 4°C. c. After adding 1 mL of the working solution, observe for one to three hours. c. Centrifuge at 400 g for three to four minutes at 4 °C; remove supernatant. d. Wash twice, for five minutes each time. One x 10^6 cells per milliliter. Wash for five minutes each time, twice, using PBS. e. Re-suspend cells in PBS or serum-free culture medium. either flow cytometry monitoring or fluorescence microscopy. 2.2 Adherent cells Adherent cells should be cultured on sterile glass slides (a). a. Take off the coverslip from the culture medium and use an aspirator to remove any extra. c. Add 100 μL working solution, give the cells a gentle shake to cover them fully, and leave them in place for 30 to 60 minutes. d. Use medium and wash twice in about five minutes. either flow cytometry monitoring or fluorescence microscopy. Note: Prior to staining, the cells must be resuspended if flow cytometry is being employed for detection. |
| References |
[1]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448. [2]. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285. |
| Additional Infomation | A fluorescent probe with low toxicity which is a potent substrate for ATP BINDING CASSETTE TRANSPORTER, SUBFAMILY B, MEMBER 1 and the bacterial multidrug efflux transporter. It is used to assess mitochondrial bioenergetics in living cells and to measure the efflux activity of ATP BINDING CASSETTE TRANSPORTER, SUBFAMILY B, MEMBER 1 in both normal and malignant cells. (Leukemia 1997;11(7):1124-30) |
Solubility Data
| Solubility (In Vitro) | DMSO : ~62.5 mg/mL (~164.12 mM) |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |