CPI-1189 is a TNF-α release inhibitor (antagonist) with antioxidant and neuro-protective (neuro-protection) activities. CPI-1189 may be utilized to study HIV-related neurotoxicity, so CPI-1189 is a candidate active molecule for neuroprotection studies in patients with HIV-related CNS diseases.

Physicochemical Properties

Molecular Formula	C13H18N2O2
Molecular Weight	234.299
Exact Mass	234.136
Elemental Analysis	C, 66.64; H, 7.74; N, 11.96; O, 13.66
CAS #	183619-38-7
Related CAS #	183619-38-7
PubChem CID	151118
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	458.3±28.0 °C at 760 mmHg
Flash Point	183.9±24.2 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.553
LogP	1.38
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	3
Heavy Atom Count	17
Complexity	286
Defined Atom Stereocenter Count	0
SMILES	CC(=O)NC1=CC=C(C=C1)C(=O)NC(C)(C)C
InChi Key	DJKNRCWSXSZACF-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H18N2O2/c1-9(16)14-11-7-5-10(6-8-11)12(17)15-13(2,3)4/h5-8H,1-4H3,(H,14,16)(H,15,17)
Chemical Name	4-acetamido-N-tert-butylbenzamide
Synonyms	CPI-1189; REN 1189; REN 1654; REN-1189; REN1654; REN-1654; REN1189; CPI1189; CPI 1189
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	TNF-alpha
References	[1]. Müller T. CPI-1189. Centaur. Curr Opin Investig Drugs. 2002 Dec;3(12):1763-7. [2]. A randomized clinical trial of CPI-1189 for HIV-associated cognitive-motor impairment. Neurology. 2002 Nov 26;59(10):1568-73. [3]. CPI-1189 protects neuronal cells from oxygen glucose deprivation/re-oxygenation-induced oxidative injury and cell death. Aging (Albany NY). 2021 Feb 17;13(5):6712-6723. [4]. CPI-1189 inhibits interleukin 1beta-induced p38-mitogen-activated protein kinase phosphorylation: an explanation for its neuroprotective properties? Neurosci Lett. 2000 Mar 10;281(2-3):179-82. [5]. Preventive actions of a synthetic antioxidant in a novel animal model of AIDS dementia. Brain Res. 1998 Jun 8;795(1-2):349-57.
Additional Infomation	CPI-1189 has been used in trials studying the treatment of HIV Infections and AIDS Dementia Complex.

Solubility Data

Solubility (In Vitro)

DMSO: 47~100 mg/mL (200.6~426.8 mM) Ethanol: ~15 mg/mL (~64.0 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2680 mL	21.3402 mL	42.6803 mL
	5 mM	0.8536 mL	4.2680 mL	8.5361 mL
	10 mM	0.4268 mL	2.1340 mL	4.2680 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.