Pomalidomide-PEG4-COOH is a ligand (for E3 ligase)-linker conjugate. Pomalidomide-PEG4-COOH contains a Pomalidomide-based cereblon ligand and a 4-unit PEG linker for PROTAC technology (disclosed in patent WO2017184995A1).

Physicochemical Properties

Molecular Formula	C24H31N3O10
Molecular Weight	521.5170
Exact Mass	521.2
CAS #	2138440-81-8
PubChem CID	134589785
Appearance	Light yellow to yellow viscous liquid
Density	1.4±0.1 g/cm3
Boiling Point	773.6±60.0 °C at 760 mmHg
Flash Point	421.6±32.9 °C
Vapour Pressure	0.0±2.8 mmHg at 25°C
Index of Refraction	1.589
LogP	-1.75
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	17
Heavy Atom Count	37
Complexity	823
Defined Atom Stereocenter Count	0
SMILES	O=C1C([H])(C([H])([H])C([H])([H])C(N1[H])=O)N1C(C2C([H])=C([H])C([H])=C(C=2C1=O)N([H])C([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])C(=O)O[H])=O
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Compounds that induce degradation of anti-apoptotic bcl-2 family proteins and the uses thereof. Patent WO2017184995A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~191.75 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (4.79 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.79 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9175 mL	9.5874 mL	19.1747 mL
	5 mM	0.3835 mL	1.9175 mL	3.8349 mL
	10 mM	0.1917 mL	0.9587 mL	1.9175 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.